NatureMethods騫村害鎶鏈細(xì)鍗曠矑瀛愪綆娓╃數(shù)瀛愭樉寰暅
2016-2-3 鏉ユ簮:涓浗鑱氬悎鐗╃綉 鐐瑰嚮嬈?/span>
鍏抽敭璇嶏細(xì)鍗曠矑瀛?/a> 浣庢俯 鐢?shù)瀛愭槕志?/a>
鏃惰繎宀佹湯錛屽悇澶ф潅蹇楁帴榪炶繘琛屼簡(jiǎn)騫寸粓鐩樼偣錛?2鏈?0鏃ョ殑銆奛atureMethods銆嬩篃鐩樼偣浜?jiǎn)骞村害鎶鏈紝閫夊嚭浜?015騫存渶鍙楀叧娉紝褰卞搷騫挎硾鐨勬妧鏈垚鏋滐細(xì)鍗曠矑瀛愪綆娓╃數(shù)瀛愭樉寰暅(cryo-EM)銆?/span>
涓涓泲鐧借川鎴栬泲鐧借川澶嶅悎鐗╃殑涓夌淮緇撴瀯鍙互鎻愪緵鏈夊叧鍏剁敓鐗╁鍔熻兘鐨勯噸瑕佽瑙ｃ備綔涓轟竴縐嶇粨鏋勬祴瀹氭妧鏈紝鍗曠矑瀛恈ryo-EM鐨勪綅嬈′粎灞呬簬楂樺垎杈ㄧ巼鏂規(guī)硶X-灝勭嚎鏅朵綋瀛﹀強(qiáng)鏍哥鍏辨尟(NMR)鍏夎氨娉曚箣鍚庛傜敱浜庤繎騫存潵鐨勬妧鏈繘灞曚嬌寰楃幇鍦ㄨ兘澶熷埄鐢╟ryo-EM瑙ｆ瀽榪戝師瀛愬垎杈ㄧ巼緇撴瀯錛岃繖縐嶆儏鍐墊鍦ㄨ繀閫熷湴鍙戠敓鏀瑰彉銆?/span>
鏁板崄騫撮噷錛孹-灝勭嚎鏅朵綋瀛︿竴鐩存槸瑙ｆ瀽铔嬬櫧璐ㄧ粨鏋勭殑棣栭夋柟娉曘傜劧鑰岋紝璁稿鐨勮泲鐧借川錛屽挨鍏舵槸鑶滆泲鐧藉拰铔嬬櫧璐ㄥ鍚堢墿鍗撮毦浠ョ粨鏅躲備竴浜涙浛浠ｄ紶緇熸櫠浣撳鐨勬柟娉曞瓨鍦ㄥ悇鑷笉鍚岀殑灞闄愭с備緥濡傦紝涓茶椋炵嬋鍏夋櫠浣撳(serialfemtosecondcrystallography)鎶鏈埄鐢ㄤ簡(jiǎn)X灝勭嚎鑷敱鐢?shù)瀛悑葊鍏?XFEL)錛屼笉鍐嶈姹傚崟涓澶ц泲鐧界粨鏅惰屾槸鑾峰緱澶ч噺瀹規(guī)槗鐢熸垚鐨勫井鏅朵綋錛岀劧鑰屽湪楂樺害涓撲笟鍖栫殑XFEL涓嬪厜鏉熺嚎鏃墮棿(beamtime)鐨勭珵浜夋槸闈炲父嬋鐑堢殑銆侼MR鍏夎氨娉曞彲鐢ㄦ潵瑙ｆ瀽灝忚泲鐧界殑緇撴瀯錛屼絾浠嶇劧闅句互灝嗗叾搴旂敤浜庤緝澶х殑铔嬬櫧銆?/span>
涓嶅悓浜庢櫠浣撳錛宑ryo-EM灝ゅ叾閫傚疁浜庤幏寰楀ぇ铔嬬櫧璐ㄥ鍚堢墿鍙?qiáng)鏄窘C哄縐嶆瀯璞℃垨緇勬垚鐘舵佺殑涓浜涚郴緇熺殑緇撴瀯淇℃伅銆傝繃鍘葷殑鏁板崄騫達(dá)紝鍦ㄨ繖涓鏈鍒濆緢灝忕殑棰嗗煙涓殑鐮旂┒浜哄憳?shù)竴鐩村湪紼蟲(chóng)鍦板墠榪涳紝鎻愰珮cryo-EM鐨勫垎杈ㄧ巼騫惰繘鑰屾墿澶у畠鐨勭敓鐗╅傚簲鎬с侲vaNogales鍦ㄦ湰鏈熺殑NatureMethods鏉傚織涓婁粙緇嶄簡(jiǎn)cryo-EM鎴愪負(fù)涓縐嶄富嫻佺粨鏋勭敓鐗╁鎶鏈殑寮鍙戝彶銆?/span>
榪欎竴鏇劇粡寰堝皬鐨勯鍩熺幇姝ｅ湪紿侀鐚涜繘銆備竴縐嶆柊鍨嬬殑楂樺害鏁忔劅鐩存帴鎺㈡祴鐓х浉鏈哄彲鐩存帴鎹曟崏鍒扮數(shù)瀛愶紝鏈夊彲鑳藉疄鐜板垎杈ㄧ巼鐨勯璺冦傜涓鎵規(guī)帰绱㈣繖浜涙柊鍨嬫帰嫻嬪櫒鐨勮鏂囧彂甯冧簬2013騫達(dá)紝2014騫寸湅鍒頒簡(jiǎn)鍑犵瘒鐢╟ryo-EM瑙ｆ瀽涓浜涢噸瑕佺殑楂樺垎杈ㄧ巼緇撴瀯鐨勮鏂囥?015騫達(dá)紝澶氶」鐮旂┒宸茬獊鐮翠簡(jiǎn)3鍩冪殑鍒嗚鯨鐜囬殰紕嶁斺旇繖涓鍓嶆墍鏈湁鐨勫．涓劇敋鑷寵涓浜涢暱鏈熺殑cryo-EM浠庝笟鑰呴兘鎰熷埌鎯婅銆?/span>
浣嗕竴鍙板ソ鐨勬嫻嬪櫒騫墮潪鏄竾鑳界殑銆備竴欏規(guī)垚鍔熺殑cryo-EM鐮旂┒寰堝ぇ紼嬪害涓婁緷璧栦簬濂界殑鏍鋒湰鍒跺浠ュ強(qiáng)澶嶆潅鐨勫浘鍍忓鐞嗚蔣浠躲侫llisonDoerr鍦ㄦ湰鏈熺殑NatureMethods鏉傚織涓婃帰璁ㄤ簡(jiǎn)榪欎竴闂銆?/span>
cryo-EM鍒嗚鯨鐜囧彉闈╂墠鍒氬垰寮濮嬶紝RobertGlaeser鍦ㄤ竴綃囪瘎璁烘枃绔犱腑瀵規(guī)榪涜浜?jiǎn)鎺㈣銆傛嫻嬪櫒鎶鏈晱鎰熷害澧為珮?shù)负寮鍙戝嚭涓浜涙柊鐨勬敼鑹柟娉曞甫鏉ヤ簡(jiǎn)鏈轟細(xì)錛岃繖浜涙柟娉曞皢榪涗竴姝ユ帹鍔ㄦ彁楂樺垎杈ㄧ巼銆侀傜敤鎬у拰鏄撶敤鎬с傚敖綆ryo-EM灝ゅ叾閫傜敤浜庡ぇ鍨嬭泲鐧借川澶嶅悎鐗╋紝鍒扮洰鍓嶄負(fù)姝㈢爺絀剁殑榪欎簺铔嬬櫧璐ㄥ鍚堢墿閮戒富瑕佹槸涓浜涘鏄撴憳鍒扮殑鏋滃疄銆傚綋鍓嶈揩鍒囬渶瑕佷竴浜涘疄鐢ㄣ佸彲閲嶅鐨勩侀氱敤鏍鋒湰鍒跺鏂規(guī)硶鏉ユ墿灞昪ryo-EM鐨勯傜敤鎬э紝媯(gè)嫻嬪嚭榪勪粖涓烘鎵鏈夌粨鏋勬妧鏈棤娉曠‘瀹氱殑緇撴瀯銆傛暟鎹垎鏋愭柟娉曚篃闇瑕佽繘涓姝ョ殑鏀硅繘錛岀爺絀朵漢鍛樺笇鏈涘緱鍒頒竴浜涚畝鍗曠殑銆佸彲闈犵殑璁＄畻鏂規(guī)硶灝嗗師濮嬬殑浜岀淮鍥懼儚杞彉?shù)皋Z笁緇寸殑铔嬬櫧璐ㄧ粨鏋勶紝灝ゅ叾鐢ㄤ簬媯(gè)嫻嬪叿鏈夌粨鏋勫紓璐ㄦх殑緋葷粺銆?/span>
鍍忔墍鏈夊浜庨閫熷闀挎湡鐨勭瀛﹂鍩熶竴鏍鳳紝cryo-EM涔熸湁鎴愰暱鐨勭儲(chǔ)鎭箋傛湰鏈熺殑NatureMethods鏉傚織鐨勪竴綃囨柊闂諱笓紼胯璁轟簡(jiǎn)榪欎竴闂銆備護(hù)浜洪紦鑸炵殑鏄紝璁稿鐨勫浗瀹墮兘鍦ㄥ緩璁懼叿鏈夐珮绔華鍣ㄧ殑鍥藉鐢ㄦ埛璁炬柦錛岀劧鑰屽綋鍓嶈繖涓楂樼浠櫒渚涗笉搴旀眰銆傝澶氱爺絀朵漢鍛橀兘鐩兼湜鑳藉鍒╃敤榪欎竴鎶鏈紝浣哻ryo-EM騫墮潪鏄竴縐嶈嚜鍔ㄥ寲鎶鏈?鑷沖皯鐩墠涓嶆槸)銆傚湪鏍鋒湰鍒跺鍜屾暟鎹垎鏋愯繃紼嬩腑鏈夎澶氬鏉傜殑姝ラ錛岀爺絀朵漢鍛樺繀欏誨皬蹇?jī)鍦版纭簲鐢ㄣ佽褰曞拰楠岃瘉浠ラ伩鍏嶇姱閿欒銆傜‘淇濇柊cryo-EM浠庝笟浜哄憳鎺ュ彈閫傚綋鐨勫煿璁嚦鍏抽噸瑕併?/span>
涓嶈繃錛宑ryo-EM鐨勮繖浜涘彂灞曞茍鏈剰鍛崇潃鏅朵綋瀛︾殑緇堢粨錛孹-灝勭嚎鏅朵綋瀛﹀皢浠嶇劧鏄竴縐嶇敤浜庤В鏋愬鏄撶粨鏅剁殑铔嬬櫧璐ㄧ粨鏋勭殑寮哄ぇ鎶鏈?/span>
鍏嶈矗澹版槑錛氶儴鍒嗚祫鏂欐潵婧愪簬緗戠粶錛岃漿杞界殑鐩殑鍦ㄤ簬浼犻掓洿澶氫俊鎭強(qiáng)鍒嗕韓錛屽茍涓嶆剰鍛崇潃璧炲悓鍏惰鐐規(guī)垨璇佸疄鍏剁湡瀹炴э紝
涔熶笉鏋勬垚鍏朵粬寤鴻銆備粎鎻愪緵浜ゆ祦騫沖彴錛屼笉涓哄叾鐗堟潈璐熻矗銆傚娑夊強(qiáng)渚墊潈錛岃鑱旂郴鎴戜滑鍙?qiáng)鏃朵慨鏀规垨鍒犻櫎銆?br>閭錛歩nfo@polymer.cn
錛堣矗浠葷紪杈戯細(xì)xu錛?
